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The prevention of autolysis of stored cornea using steroid as a lysosome membrane stabilizer.
Metadata
Journalcanadian journal of ophthalmology-journal canadien d ophtalmologie1.369Date
1975-Oct
Publication Type
Journal Article
Volume
1975-Oct / 10 : 482-6
Author
Fook TJ , Ranadive NS , Basu PK
Doi
Not Found
PMIDMESH
Acid Phosphatase
Animals
Autolysis
Cornea
Corneal Transplantation
Hydrocortisone
Hydrogen-Ion Concentration
Lysosomes
Membranes
Rabbits
Refrigeration
Temperature
Tissue Preservation
Transplantation, Homologous
Abstract
Many eyes donated for use in corneal grafting are rejected because of signs of autolysis in the donor material. The purpose of this experimental study was to determine whether hydrocortisone acting as a lysosome membrane stabilizer could prevent or retard autolysis of the corneas under storage, and if so, what was the most efficacious concentration. Different groups of rabbit corneas were placed in saline as controls or in varying concentrations of hydrocortisone (10(-10) M to 10(-4) M at pH 7.4) at 37 degrees C and 4 degrees C. Acid phosphatase released after six hours was measured biochemically. This enzyme was used as a marker enzyme reflecting lysosomal labilization. Results showed a significant stabilization of the lysosomal membrane at 4 degrees C as compared to 37 degrees C. A trend towards stabilization of the lysosomal membrane was seen when 10(-8) M concentration of hydrocortisone at 37 degrees C was used, there being no demonstrable stabilization at 4 degrees C.
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Can J Ophthalmolcanadian journal of ophthalmology-journal canadien d ophtalmologie
Metadata
LocationEngland
FromCANADIAN OPHTHAL SOC

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