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Enzymatic hydrolysis of agar: purification and characterization of neoagarobiose hydrolase and p-nitrophenyl alpha-galactoside hydrolase.
Metadata
Journalcanadian journal of microbiology1.793Date
1975-Oct
Publication Type
Journal Article
Volume
1975-Oct / 21 : 1512-8
Author
Day DF , Yaphe W
DoiPMIDMESH
Agar
Ammonium Sulfate
Cell Membrane
Cell-Free System
Chemical Precipitation
Glycoside Hydrolases
Hydrogen-Ion Concentration
Hydrolysis
Lithium
Molecular Weight
Nitrophenylgalactosides
Polysaccharides
Potassium
Pseudomonas
Seawater
Sodium
Water Microbiology
Abstract
The mixture of polysaccharides in the gelling component of agar (agarose) is hydrolyzed to D-galactose and 3,6-anhydro-L-galactose by a series of hydrolytic enzymes obtained from Pseudomonas atlantica. The final degradative step in the pathway of agarose decomposition is the hydrolysis of the alpha-linkage in the dissaccharide neoagarobiose yielding D-galactose and 3,6-anhydro-L-galactose. Pseudomonas atlantica when grown on agar produces two specific enzymes, p-nitrophenyl alpha-galactose hydrolase and neoagarobiose hydrolase. The purification and partial characterization of both enzymes are presented.
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1.8
Can J Microbiolcanadian journal of microbiology
Metadata
LocationCanada
FromCANADIAN SCIENCE PUBLISHING

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