MedicGo
Serum lactate dehydrogenase activity ratios with different substrates.
Metadata
Journalclinical biochemistry2.573Date
1975-Oct
Publication Type
Journal Article
Comparative Study
Volume
1975-Oct / 8 : 307-15
Author
Barlow D , Naghizadeh F , King J
DoiPMIDMESH
Butyrates
Glyoxylates
Humans
Hydrogen-Ion Concentration
Isoenzymes
Kinetics
L-Lactate Dehydrogenase
Lactates
Liver
Myocardium
Pyruvates
Abstract
1. The lactate dehydrogenase activity of 89 sera from patients suffering myocardial infarction and of 55 sera from patients with hepatocellular damage was assayed under optimal conditions using pyruvate, alpha-oxobutyrate, hydroxypyruvate and glyoxylate as substrates. Activity was also measured with lactate as substrate at different pH values. 2. The ratios of activities under these different assay conditions were calculated for both series of patients. Correct differentiations for single ratios ranged from virtually nil for hydroxypyruvate/alpha-oxobutyrate to is greater than 93 per cent for glyoxylate/hydroxypyruvate and glyoxylate/alpha-oxobutyrate. This was little improved by the use of multiple ratios involving up to seven separate assays. 3. The activity ratio of hydroxypyruvate to pyruvate which is consistently greater than unity was found to be inverted in a case of morphine poisoning.
Fav
Like
Download
Share
Export
Cite
2.6
Clin Biochemclinical biochemistry
Metadata
LocationUnited States
FromPERGAMON-ELSEVIER SCIENCE LTD

No Data

© 2017 - 2020 Medicgo
Powered by some medical students