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Improved high-resolution high-voltage paper electrophoresis system for use in screening for aminoacidopathies.
Metadata
Journalclinical chemistry7.292Date
1975-Dec
Publication Type
Journal Article
Comparative Study
Volume
1975-Dec / 21 : 1981-2
Author
Spiekerman AM , Hetherly ME , Hall FF
Doi
Not Found
PMIDMESH
Amino Acid Metabolism, Inborn Errors
Amino Acids
Electrophoresis, Paper
Humans
Hydrogen-Ion Concentration
Abstract
High-voltage paper electrophoresis of small samples of serum and urine at pH 6.0 resolves basic and acidic amino acids and separates them from the neutral amino acids. For separation and identification of the neutral amino acids, the appropriate area of the electrophoretogram is cut out, sewn onto a second sheet of paper, and rerun at pH 1.9. By this method, amino acids are rapidly resolved. It is suited for use with special procedures such as oxidation of biological fluid with performic acid and specific staining for confirmation of amino acid identification.
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7.3
Clin Chemclinical chemistry
Metadata
LocationEngland
FromOXFORD UNIV PRESS INC

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