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Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns.
Metadata
Journaldevelopmental cell10.092Date
2018 Nov 08
2 years ago
Publication Type
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Journal Article
Volume
2018-12-17 / 47 : 801-813.e6
Author
Ebbing A 1, Vértesy Á 1, Betist MC 1, Spanjaard B 2, Junker JP 2, Berezikov E 3, van Oudenaarden A 1, Korswagen HC 4
Affiliation
  • 2. Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, 13092 Berlin-Buch, Germany.
  • 3. European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands.
  • 4. Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands. Electronic address: [email protected]
Doi
PMIDMESH
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Cell Differentiation
Disorders of Sex Development
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental
Germ Cells
Gonads
Hermaphroditic Organisms
Male
Meiosis
Nuclear Proteins
Ovary
RNA, Messenger
Sex Characteristics
Sex Determination Processes
Spatio-Temporal Analysis
Spermatozoa
Transcription Factors
Transcriptome
Abstract
To advance our understanding of the genetic programs that drive cell and tissue specialization, it is necessary to obtain a comprehensive overview of gene expression patterns. Here, we have used spatial transcriptomics to generate high-resolution, anteroposterior gene expression maps of C. elegans males and hermaphrodites. To explore these maps, we have developed computational methods for discovering region- and tissue-specific genes. We have found extensive sex-specific gene expression differences in the germline and sperm and discovered genes that are specifically expressed in the male reproductive tract. These include a group of uncharacterized genes that encode small secreted proteins that are required for male fertility. We conclude that spatial gene expression maps provide a powerful resource for identifying tissue-specific gene functions in C. elegans. Importantly, we found that expression maps from different animals can be precisely aligned, enabling transcriptome-wide comparisons of gene expression patterns.
Keywords: C. elegans CEL-seq germline mRNA sequencing male fertility spatial transcriptomics sperm
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10.1
Dev Celldevelopmental cell
Metadata
LocationUnited States
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