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9-Plex ultra high performance liquid chromatography tandem mass spectrometry determination of free hydroxyl polycyclic aromatic hydrocarbons in human plasma and urine.
Metadata
Journaljournal of chromatography a4.049Date
2020 May 06
3 months ago
Publication Type
Journal Article
Volume
2020-Jul-19 / 1623 : 461182
Author
Sun L 1, Zhu S 2, Zheng Z 3, Sun J 4, Zhao XE 5, Liu H 6
Affiliation
  • 2. Key Laboratory of Life-Organic Analysis of Shandong Province & Key Laboratory of Pharmaceutical Intermediates and Natural Medicine Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, Shandong, PR China. Electronic address: [email protected]
  • 3. College of Food Science and Engineering, Shandong Agricultural University, Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, Taian 271018, PR China.
  • 4. Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, Qinghai, PR China.
  • 5. Key Laboratory of Life-Organic Analysis of Shandong Province & Key Laboratory of Pharmaceutical Intermediates and Natural Medicine Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, Shandong, PR China. Electronic address: [email protected]
  • 6. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
Doi
PMIDMESH
Abstract
Hydroxyl-polycyclic aromatic hydrocarbons (OH-PAHs) are biomarkers for assessing the exposure levels of polycyclic aromatic hydrocarbons (PAHs). A series of stable isotope mass tags (SIMT-332/338/346/349/351/354/360/363/374/377) were firstly designed and synthesized to perform multiplexed stable isotope labeling derivatization (MSILD) of OH-PAHs in human plasma and urine. Their derivatives were enriched and purified by magnetic dispersive solid phase extraction (MDSPE) using prepared Fe3O4/GO and then determined by ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) in multiple reaction monitoring mode. 9-Plexed MSILD reagents were prepared using pipemidic acid as core structure with different isotope mass tags, in which carbonyl chloride group was used to label OH-PAHs. The SIMT-346 labeled OH-PAHs standards were used as internal standards, which can greatly increase the quantitative accuracy. 9-Plex labeled nine different real samples can be quantified by UHPLC-MS/MS in a single run. Under optimized MSILD-MDSPE conditions, good linearities of seven OH-PAHs were obtained with satisfactory coefficient of determination R2 > 0.991. Limits of detection (LODs) of seven OH-PAHs were from 0.1 to 0.5 pg/mL, and limits of quantitation (LOQs) ranged from 0.5 to 2.0 pg/mL. The intra- and inter-day precisions ranged in 2.3-12.4% with accuracies in the range of 91.7-108.4%. Acceptable results of matrix effect (89.7-105.7%) and derivatization efficiency (> 96.4%) were obtained. In short, the developed method has been proved to be high-throughput, sensitive, accurate and easy-handling. This method was applied for the measurement of seven free OH-PAHs in human urine and plasma, and expected to provide technical support for the evaluation of PAHs exposure levels in humans.
Keywords: Derivatization Exposome High throughput Hydroxyl polycyclic aromatic hydrocarbons Magnetic dispersive solid phase extraction Multiplexed tags chemical isotope labeling
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4.0
J Chromatogr Ajournal of chromatography a
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LocationNetherlands
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