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Further study on enantiomer resolving ability of amylose tris(3-chloro-5-methylphenylcarbamate) covalently immobilized onto silica in nano-liquid chromatography and capillary electrochromatography.
Metadata
Journaljournal of chromatography a4.049Date
2020 May 08
6 months ago
Type
Journal Article
Volume
2020-Jul-19 / 1623 : 461213
Author
D'Orazio G 1, Fanali C 2, Fanali S 3, Gentili A 4, Karchkhadze M 5, Chankvetadze B 5
Affiliation
  • 2. Department of Science and Technology for Humans and the Environment, University Campus Bio-Medico of Rome, Via Alvaro del Portillo 21, 00128 Rome, Italy.
  • 3. Teaching Committee of Ph.D. School in Natural Science and Engineering, University of Verona, Strada Le Grazie, 15 - 37129 Verona, Italy. Electronic address: [email protected]
  • 4. Department of Chemistry "Sapienza" University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
  • 5. Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Iv. Javakhishvili Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia.
Doi
PMIDMESH
Acids
Amylose
Capillary Electrochromatography
Chromatography, Liquid
Flavanones
Phenylcarbamates
Silicon Dioxide
Stereoisomerism
Abstract
In the present study separation of enantiomers of some chiral neutral, basic and weakly acidic analytes was investigated on the chiral stationary phase (CSP) made by covalent immobilization of amylose tris(3-chloro-5-methylphenylcarbamate) onto aminopropylsilanized (APS) silica in nano-liquid chromatography (nano-LC) in aqueous methanol or acetonitrile mixtures. It has been shown that similar to high-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) this chiral selector is useful for separation of enantiomers of neutral, basic and acidic analytes also in nano-LC. In comparison to our previous research, in which the chiral selector (CS) was bonded on native silica, in this study, the CS was immobilized on APS silica in order to improve chromatographic performance towards basic analytes. In fact, some improvement was observed and surprisingly not only for basic but also for neutral and acidic analytes. Again, quite unexpectedly almost no electroosmotic flow (EOF) was observed in capillaries packed with ca. 20% (w/w) amylose tris(3-chloro-5-methylphenylcarbamate) immobilized onto APS silica although the same APS silica before attachment of chiral selector exhibited significant EOF. In order to generate EOF in the capillaries with the CSP and enable capillary electrochromatographic (CEC) experiment on it, the short segment of the capillary column was packed with APS silica without chiral selector. The EOF in such capillary enabled CEC experiment and some preliminary results are reported here.
Keywords: Amylose tris(3-chloro-5-methylphenylcarbamate) Capillary electrochromatography Covalently immobilized polysaccharide-based chiral stationary phase Enantioseparations nano-Liquid Chromatography
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J Chromatogr Ajournal of chromatography a
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