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Phosphorylation of mitochondrial transcription factor B2 controls mitochondrial DNA binding and transcription.
Metadata
Journalbiochemical and biophysical research communications2.985Date
2020 Jun 03
5 months ago
Type
Journal Article
Volume
2020-Jul-30 / 528 : 580-585
Author
Bostwick AM 1, Moya GE 1, Senti ML 1, Basu U 2, Shen J 2, Patel SS 2, Dittenhafer-Reed KE 3
Affiliation
  • 2. Rutgers University, Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, 683 Hoes Lane, Piscataway, NJ, 08854, United States.
  • 3. Hope College, Department of Chemistry, 35 E. 12th Street, Holland, MI, 49423, United States. Electronic address: [email protected]
Doi
PMIDMESH
Abstract
Mammalian cells contain genetic information in two compartments, the nucleus and the mitochondria. Mitochondrial gene expression must be coordinated with nuclear gene expression to respond to cellular energetic needs. To gain insight into the coordination between the nucleus and mitochondria, there is a need to understand the regulation of transcription of mitochondrial DNA (mtDNA). Reversible protein post-translational modifications of the mtDNA transcriptional machinery may be one way to control mtDNA transcription. Here we focus on a member of the mtDNA transcription initiation complex, mitochondrial transcription factor B2 (TFB2M). TFB2M melts mtDNA at the promoter to allow the RNA polymerase (POLRMT) to access the DNA template and initiate transcription. Three phosphorylation sites have been previously identified on TFB2M by mass spectrometry: threonine 184, serine 197, and threonine 313. Phosphomimetics were established at these positions. Proteins were purified and analyzed for their ability to bind mtDNA and initiate transcription in vitro. Our results indicate phosphorylation at threonine 184 and threonine 313 impairs promoter binding and prevents transcription. These findings provide a potential regulatory mechanism of mtDNA transcription and help clarify the importance of protein post-translational modifications in mitochondrial function.
Keywords: Mitochondrial DNA Mitochondrial transcription factor B2 (TFB2M) Phosphorylation Transcription regulation
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Biochem Biophys Res Communbiochemical and biophysical research communications
Metadata
LocationUnited States
FromACADEMIC PRESS INC ELSEVIER SCIENCE

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