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Levels of the neuropeptide phoenixin-14 and its receptor GRP173 in the hypothalamus, ovary and periovarian adipose tissue in rat model of polycystic ovary syndrome.
Metadata
Journalbiochemical and biophysical research communications2.985Date
2020 Jun 03
4 months ago
Type
Journal Article
Volume
2020-Aug-06 / 528 : 628-635
Author
Kalamon N 1, Błaszczyk K 1, Szlaga A 2, Billert M 3, Skrzypski M 3, Pawlicki P 4, Górowska-Wójtowicz E 4, Kotula-Balak M 5, Błasiak A 2, Rak A 6
Affiliation
  • 2. Departament of Neurophysiology and Chronobiology, Institute of Zoology and Biomedical Research, Jagiellonian University, Poland.
  • 3. Department of Animal Physiology, Biochemistry and Biostructure, Poznań University of Life Sciences, Poland.
  • 4. Departament of Endocrinology, Institute of Zoology and Biomedical Research, Jagiellonian University, Poland.
  • 5. University Centre of Veterinary Medicine JU-AU, University of Agriculture in Kraków, Poland.
  • 6. Departament of Physiology and Toxicology of Reproduction, Institute of Zoology and Biomedical Research, Jagiellonian University, Poland. Electronic address: [email protected]
Doi
PMIDMESH
Abstract
Phoenixin (PNX) is a newly discovered peptide produced by proteolytic cleavage of a small integral membrane protein 20 (Smim20), which acts as an important regulator of energy homeostasis and reproduction. Since dysfunction of reproduction is characteristic in polycystic ovarian syndrome (PCOS), the role of PNX in pathogenesis of PCOS needs further investigation. The objective of this study was to determine expression of Smim20, PNX-14 and its receptor GRP173 in the hypothalamus, ovary and periovarian adipose tissue (PAT) of letrozole induced PCOS rats. Phosphorylation of extracellular signal-regulated kinase (ERK1/2), protein kinases A (PKA) and B (Akt) were also estimated. We observed that PCOS rats had high weight gain and a number of ovarian cyst, high levels of testosterone, luteinizing hormone and PNX-14, while low estradiol. Smim20 mRNA expression was higher in the ovary and PAT, while PNX-14 peptide production was higher only in the ovary of PCOS rat. Moreover, in PCOS rats Gpr173 level was lower in PAT but at the protein level increased only in the ovary. Depending on the tissues, kinases phosphorylation were significantly differ in PCOS rats. Our results showed higher levels of PNX-14 in PCOS rats and indicated some novel findings regarding the mechanisms of PCOS pathophysiology.
Keywords: Adipose tissue Hypothalamus Ovary Phoenixin-14 Polycystic ovary syndrome
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Biochem Biophys Res Communbiochemical and biophysical research communications
Metadata
LocationUnited States
FromACADEMIC PRESS INC ELSEVIER SCIENCE

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