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Electricity-free amplification and visual detection of Cronobacter species in powdered infant formula.
Metadata
Journaljournal of dairy science3.333Date
2020 Jun 03
4 months ago
Type
Journal Article
Volume
2020-Aug / 103 : 6882-6893
Author
Fu S 1, Jiang Y 1, Qin X 1, Yang T 1, Chen S 1, Yang X 1, Zhang W 1, Qu Y 1, Man C 2
Affiliation
  • 2. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: [email protected]
Doi
PMIDMESH
Abstract
Due to the lack of electricity and thermostatic instruments in certain settings for convenient detection of Cronobacter species in powdered infant formula (PIF), a novel investigation was conducted to establish an electricity-free visual detection system for rapid detection of Cronobacter species in PIF. This system included a portable electricity-free heater that could use the exothermic reaction of calcium oxide and water and 3 kinds of phase change materials to supply 3 constant temperatures for immunomagnetic separation, DNA extraction, and loop-mediated isothermal amplification assay. Meanwhile, the amplified reaction combined with hydroxynaphthol blue could achieve rapid visual detection. Primers designed based on the 16S-23S ribosomal RNA internal transcribed spacer were used in loop-mediated isothermal amplification to specifically monitor Cronobacter species, and the detection limit can reach 4.2 × 102 cfu/g in PIF by an electricity-free heater in 2 h 30 min. Moreover, 2 h of pre-enrichment was necessary when the level of the PIF samples with Cronobacter spp. was 100 cfu/g. The stability of the system was evaluated in ambient temperature at 4°C, 25°C, and 37°C. The results suggested that the electricity-free heater can maintain 3 constant temperatures to support different processes. Therefore, this amplification and visual system is applicable for use in many fields for rapid and specific detection of Cronobacter species in PIF.
Keywords: Cronobacter species electricity-free visual detection immunomagnetic separation loop-mediated isothermal amplification powdered infant formula
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J Dairy Scijournal of dairy science
Metadata
LocationUnited States
FromELSEVIER SCIENCE INC

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