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A cytokine receptor domeless promotes white spot syndrome virus infection via JAK/STAT signaling pathway in red claw crayfish Cherax quadricarinatus.
Metadata
Journaldevelopmental and comparative immunology3.192Date
2020 Jun 04
4 months ago
Type
Journal Article
Volume
2020-Oct / 111 : 103749
Author
Liu LK 1, Chen XX 2, Gao RL 1, Wang KJ 1, Zheng WY 3, Liu HP 4
Affiliation
  • 2. State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen, 361102, Fujian, PR China; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, PR China.
  • 3. Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, PR China. Electronic address: [email protected]
  • 4. State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen, 361102, Fujian, PR China. Electronic address: [email protected]
Doi
PMIDMESH
Abstract
The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway is pivotal in immune responses for a variety of pathogens in both vertebrates and invertebrates. Domeless (Dome), as a unique cytokine receptor, involves in the upstream JAK/STAT pathway in invertebrates. In this study, the full-length cDNA sequence of a cytokine receptor Dome was identified from red claw crayfish Cherax quadricarinatus (named as CqDome), which contained an open reading frame of 4251 bp, encoding 1416 amino acids. The CqDome contained extracellular conservative domains of a signal peptide, two cytokine binding modules (CBM), three fibronectin-type-III-like (FN3) domains and a transmembrane region. Tissue distribution analysis showed that CqDome generally expressed in all the tissues selected with a high expression in hemocyte. The gene expression of both the viral immediately early gene (IE1) and a late gene envelope protein VP28 of white spot syndrome virus (WSSV) were significantly decreased after gene silencing of CqDome in crayfish haematopoietic tissue (Hpt) cells, indicating a key role of CqDome in promoting WSSV infection. Furthermore, the phosphorylation level of CqSTAT was significantly inhibited by gene silencing of CqDome in Hpt cells, indicating that CqDome participated in signal transduction of JAK/STAT pathway in red claw crayfish. These data together suggest that CqDome is likely to promote WSSV infection via JAK/STAT pathway, which sheds new light on further elucidation of the pathogenesis of WSSV.
Keywords: Cherax quadricarinatus Domeless JAK/STAT pathway Phosphorylation White spot syndrome virus
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Dev Comp Immunoldevelopmental and comparative immunology
Metadata
LocationUnited States
FromELSEVIER SCI LTD

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