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Immobilization of laccase on Sepharose-linked antibody support for decolourization of phenol red.
Metadata
Journalinternational journal of biological macromolecules5.162Date
2020 Jun 04
5 months ago
Type
Journal Article
Volume
2020-Oct-15 / 161 : 78-87
Author
Zofair SFF 1, Arsalan A 1, Khan MA 2, Alhumaydhi FA 3, Younus H 4
Affiliation
  • 2. Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia.
  • 3. Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia.
  • 4. Enzymology Laboratory, Interdisciplinary Biotechnology Unit, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India. Electronic address: [email protected]
Doi
PMIDMESH
Abstract
Laccases which are considered as "green tools" in biotechnology have potential to degrade toxic contaminants/synthetic dyes present in industrial effluents. The loss in activity and stability of laccases are key challenges faced in their potential industrial applications. Here, laccase from Trametes versicolor (polypore mushroom) was immobilized on Sepharose-linked antibody support to carry out the decolourization of phenol red. This support was prepared by covalent linking of anti-laccase antibodies to CNBr activated Sepharose at pH 8.5, and then laccase was immobilized on this affinity support at pH 5.0. The amount of laccase immobilized was approximately 33 mg per gram of the affinity support, giving an immobilization yield of 83.4%. The immobilized enzyme displayed an activity of 3.88 U with an effectiveness factor (η) of 0.90. Immobilization of laccase led to significant enhancement in thermal and storage stability. The immobilized enzyme retained 44% of its activity after 10 cycles of continuous use. The decolourization of phenol red dye obtained by immobilized and soluble laccase after 6 h of incubation at 50 °C was 80 and 56%, respectively. Thus, immobilization of laccase on Sepharose-linked antibody support leads to remarkable improvement in its various properties, making it more versatile for industrial applications.
Keywords: Antibody support Immobilization Laccase
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Int J Biol Macromolinternational journal of biological macromolecules
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