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Regeneration of Tooth with Allogenous, Autoclaved Treated Dentin Matrix with Dental Pulpal Stem Cells: An In Vivo Study.
Metadata
Journaljournal of endodontics3.118Date
2020 Jun 04
4 months ago
Type
Journal Article
Volume
2020-Sep / 46 : 1256-1264
Author
Chang CC 1, Lin TA 2, Wu SY 1, Lin CP 3, Chang HH 4
Affiliation
  • 2. Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
  • 3. Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
  • 4. Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan. Electronic address: [email protected]
Doi
PMIDMESH
Animals
Cell Differentiation
Cells, Cultured
Dental Pulp
Dentin
Mice
Regeneration
Stem Cells
Sterilization
Tissue Scaffolds
Abstract
INTRODUCTION: Biomaterials designed for tissue engineering should be nontoxic and nonimmunogenic and should achieve their intended functions. Treated dentin matrix (TDM), a bioactive extracellular matrix, is promising for tooth regeneration. However, the effect of sterilization on the surface properties of allogenous TDM in the animal model is unclear.
METHODS: The biological characteristics and influences of dental pulp stem cells (DPSCs) with autoclaved TDM (a-TDM) were studied using scanning electron microscopy, immunofluorescence microscopy, immunohistochemistry, and reverse transcription polymerase chain reaction in vitro. In addition, a-TDM was implanted in a mouse model for 6 weeks and was a substrate with DPSCs for tooth reconstruction in a goat animal model in vivo.
RESULTS: Allogenous a-TDM induced and supported DPSCs to develop new dentin pulp-like tissues, enamel dental pulp, and cementum periodontal complexes. Immunohistochemistry data showed that the markers dentin sialoprotein, βⅢ-tubulin, dentin matrix protein 1, amelogenin, VIII factors, type I collagen, cementum-derived attachment protein, and scleraxis transcription factor were positive stained in toothlike tissue.
CONCLUSIONS: Allogenous a-TDM served as an effective scaffold enabling DPSCs to proliferate and differentiate into a broad array of resident cells including odontoblasts, fibroblasts, vascular cells, and neural endings. Allogenous a-TDM with DPSCs can provide an ideal biomaterial for optimizing the regeneration of tooth material.
Keywords: Autoclaved dental pulp stem cells in vivo study tissue engineering treated dentin matrix
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3.1
J Endodjournal of endodontics
Metadata
LocationUnited States
FromELSEVIER SCIENCE INC

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