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Identification of cross-reactive human single-chain variable fragments against phospholipases A2 from Lachesis muta and Bothrops spp venoms.
Metadata
Journaltoxicon2.201Date
2020 Jun 04
4 months ago
Type
Journal Article
Volume
2020-Sep / 184 : 116-121
Author
Campos LB 1, Pucca MB 2, Silva LC 3, Pessenda G 1, Filardi BA 1, Cerni FA 4, Oliveira IS 5, Laustsen AH 6, Arantes EC 5, Barbosa JE 1
Affiliation
  • 2. Medical School, Federal University of Roraima, Boa Vista, RR, Brazil; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark. Electronic address: [email protected]
  • 3. Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil; Toronto Recombinant Antibody Centre, The Donnelly Centre, University of Toronto, Toronto, Canada.
  • 4. Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark; Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
  • 5. Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
  • 6. Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
Doi
PMIDMESH
Animals
Antivenins
Bothrops
Crotalid Venoms
Humans
Single-Chain Antibodies
Snake Bites
Viperidae
Abstract
Bushmasters (Lachesis spp) and lancehead vipers (Bothrops spp) are two of the most dangerous snakes found in Latin America. Victims of envenoming by these snakes require urgent administration of antivenom. Here, we report the identification of a small set of broadly neutralizing human monoclonal single-chain variable fragment (scFv) antibodies targeting key phospholipases A2 from Lachesis and Bothrops spp using phage display technology and demonstrate their in vitro efficacy using a hemolysis assay.
Keywords: Antivenom Bothrops spp Cross-reactive antibodies Lachesis muta Monoclonal antibody Phage display
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Toxicontoxicon
Metadata
LocationEngland
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