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A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy.
Metadata
Journaljournal of pharmacological sciences2.835Date
2020 May 11
5 months ago
Type
Journal Article
Volume
2020-Aug / 143 : 320-324
Author
Miyano K 1, Ohbuchi K 2, Sudo Y 3, Minami K 4, Yokoyama T 4, Yamamoto M 2, Uzu M 1, Nonaka M 1, Shiraishi S 1, Murata H 5, Higami Y 6, Uezono Y 7
Affiliation
  • 2. Tsumura Research Labs, Tumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, Japan.
  • 3. Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan.
  • 4. Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Emergency Life-Saving Technique Academy of Tokyo (ELSTA TOKYO), Hachioji-shi, Tokyo 192-0364, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, Tochigi 329-0498, Japan.
  • 5. Department of Anesthesiology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan.
  • 6. Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan.
  • 7. Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan; Division of Supportive Care Research, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Tokyo 104-0045, Japan; Innovation Center for Supportive, Palliative and Psychosocial Care, National Cancer Center Hospital, Tokyo 104-0045, Japan; Department of Comprehensive Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan. Electronic address: [email protected]
Doi
PMIDMESH
Dielectric Spectroscopy
HEK293 Cells
Humans
TRPA1 Cation Channel
TRPV Cation Channels
Transient Receptor Potential Channels
Abstract
Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC50) and efficacy (Emax) of these agonists with CellKey™ were almost identical to those made using the traditional Ca2+ influx assay in TRPV1- or TRPA1-expressing cells, suggesting that CellKey™ is a simpler and easier means of evaluating TRP activities.
Keywords: CellKey™ system Cellular dielectric spectroscopy TRPV1
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2.8
J Pharmacol Scijournal of pharmacological sciences
Metadata
LocationJapan
FromJAPANESE PHARMACOLOGICAL SOC

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