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1H NMR-based metabolomics analyses in children with Helicobacter pylori infection and the alteration of serum metabolites after treatment.
Metadata
Journalmicrobial pathogenesis2.914Date
2020 Jun 04
4 months ago
Type
Journal Article
Volume
2020-Jun-04 / 147 : 104292
Author
Fang LJ 1, Lin XC 1, Huang D 2, Pan TT 1, Yan XM 1, Hu WG 1, Zhu H 1, Xu Z 1, Zhu XZ 1, Lu HJ 1, Chen GP 1, Huang KY 3
Affiliation
  • 2. The Second School of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
  • 3. Department of Pediatric Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China. Electronic address: [email protected]
Doi
PMIDMESH
Abstract
BACKGROUND AND AIMS: Helicobacter pylori (H. pylori) infection can occur in early childhood, without eradication therapies such infection can persist throughout life and cause many different diseases. This study investigated the metabolic characteristics and explored the underlying mechanism of children with H. pylori infection, and identified potential biomarkers for evaluating the efficacy of H. pylori eradication therapies.
METHODS: We performed 1H NMR-based metabonomics coupled with multivariate analysis to investigate the metabolic profiling of serum samples between Children with and without H. pylori infection. In the same manner, we compared the alternations of metabolites in H. pylori-infected children before and after H. pylori eradication therapies.
RESULTS: 21 metabolites from serum in H. pylori-infected and H. pylori-uninfected children were identified, which were mainly involved in energy, amino acid, lipid and microbial metabolism. We found that the serum levels of trimethylamine N-oxide and alanine were significantly higher in H. pylori-infected children compared to uninfected sera, whereas lactate was significantly lower. We also found that the levels of trimethylamine N-oxide and creatine in H. pylori-infected children was significantly decreased after H. pylori eradication therapies, whereas lactate and low-density lipoprotein/very low-density lipoprotein was significantly increased.
CONCLUSIONS: This is the first study using 1H NMR-based metabolomics approach to explore the effects of H. pylori infection in children. Our results demonstrated that the disturbances of metabolism in energy, amino acids, lipids and microbiota could play an important role in the pathogenesis of gastrointestinal and extragastric diseases caused by H. pylori infection. Trimethylamine N-oxide and lactate might serve as potential serum biomarkers for evaluating the efficacy of H. pylori eradication therapies.
Keywords: Biomarker Children Helicobacter pylori Metabolite Metabolomics NMR
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2.9
Microb Pathogmicrobial pathogenesis
Metadata
LocationEngland
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