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Colorimetric biosensing of nopaline synthase terminator using [email protected] and hemin-functionalized reduced graphene oxide.
Metadata
Journalanalytical biochemistry2.877Date
2020 Jun 04
4 months ago
Type
Research Support, Non-U.S. Gov't
Journal Article
Volume
2020-08-01 / 602 : 113798
Author
Cao X 1, Xia Z 1, Yan W 1, He S 1, Xu X 2, Wei Z 1, Ye Y 3, Zheng H 4
Affiliation
  • 2. College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, 211816, China.
  • 3. School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, China. Electronic address: [email protected]
  • 4. Technology Center of Hefei Customs, Hefei, 230032, China.
Doi
PMIDMESH
Abstract
In this paper, we present a simple and label-free colorimetric biosensor for detection of the nopaline synthase (NOS) terminator in genetically modified (GM) plants. The "signal on" colorimetric biosensor was developed using a nanocomposite consisted of gold nanoparticles doped magnetic Fe3O4 nanoparticles ([email protected] NP), capture probe DNA (cDNA), and hemin-functionalized reduced graphene oxide nanosheets (H-GN). The nanocomposite was successfully prepared by means of Au-S bonds and the strong π interactions between cDNA and H-GN. The sensing approach is based on the excellent peroxidase-mimicking activity of H-GN and its different electrostatic interactions with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). In presence of the target NOS, the cDNA in the nanocomposite will hybridize with its complementary sequence, and form dsDNA structure. Due to the weak π interactions between dsDNA and H-GN, a portion of H-GN will be released from the surface of [email protected] NPs and transferred into solution. After magnetic separation was performed, the supernatant was incubated with 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2. The released H-GN can catalyze the oxidation reaction of TMB and turn the colorless solution blue. This "signal-on" colorimetric biosensor shows a broad linear range of 0.5-100 nM for the target NOS, with a 0.19 nM detection limit. The application of the biosensor for determination of NOS segments in samples of GM and non-GM tomatoes shows that it can discriminate between GM and non-GM plants. The reliability of the method for samples of NOS-spiked GM tomato suggests satisfactory recoveries in the range of 93.6%-94.2%.
Keywords: Colorimetric biosensor Genetically modified (GM) plants Label-free Peroxidase-mimicking activity
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2.9
Anal Biochemanalytical biochemistry
Metadata
LocationUnited States
FromACADEMIC PRESS INC ELSEVIER SCIENCE

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