MedicGo
Poly(8-aminoguanylic acid): formation of ordered self-structures and interaction with poly(cytidylic acid).
Metadata
Journalbiochemistry2.865Date
1975-Nov-18
Publication Type
Journal Article
Volume
1975-Nov-18 / 14 : 5033-45
Author
Hattori M , Frazier J , Miles HT
DoiPMIDMESH
Binding Sites
Circular Dichroism
Drug Stability
Escherichia coli
Hydrogen Bonding
Hydrogen-Ion Concentration
Micrococcus
Models, Molecular
Nucleic Acid Conformation
Poly C
Poly G
Polyribonucleotide Nucleotidyltransferase
Polyribonucleotides
Spectrophotometry, Infrared
Spectrophotometry, Ultraviolet
Temperature
Abstract
Poly(8-aminoguanylic acid) has in neutral solution a novel ordered structure of high stability. The 8-amino group permits formation of three hydrogen bonds between two residues along the "top", or long axis, of the purines. The usual hydrogen bonding protons and Watson-Crick pairing sites are not involved in the association. The bonding scheme has a twofold rotation axis and is hemiprotonated at N(7). Poly(8NH2G) is converted by alkaline titration (pK = 9.7) to a quite different ordered structure, which is the favored form over the range approximately pH 10-11. The bonding scheme appears to be composed of a planar, tetrameric array of guanine residues, in which the 8-amino group does not participate in interbase hydrogen bonding. Poly (8NH2G) does not interact with poly(C) in neutral solution because of the high stability of the hemiprotonated G-G self-structure. Titration to the alkaline plateau, however, permits ready formation of a two-stranded Watson-Crick helix. In contrast to the monomer 8NH2GMP, poly(8NH2G) does not form a triple helix with poly(C) under any conditions. The properties of the ordered structures are interpreted in terms of a strong tendency of the 8-amino group to form a third interbase hydrogen bond, when this possibility is not prevented by high pH.
Fav
Like
Download
Share
Export
Cite
2.9
Biochemistrybiochemistry
Metadata
LocationUnited States
FromAMER CHEMICAL SOC

No Data

© 2017 - 2020 Medicgo
Powered by some medical students