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The pH dependence of the reactions of flavin triplet states with amino acids. A laser flash photolysis study.
Metadata
JournalBiochim. Biophys. ActaNot FoundDate
1979-Oct-18
Type
Journal Article
Volume
1979-Oct-18 / 587 : 455-62
Author
Heelis PF , Parsons BJ , Phillips GO
DoiPMIDMESH
Amino Acids
Chemical Phenomena
Chemistry
Edetic Acid
Electron Transport
Flavin Mononucleotide
Hydrogen-Ion Concentration
Lasers
Photolysis
Abstract
The pH dependence of the rate constants of reaction of several amino acids with the triplet states of flavin mononucleotide in aqueous solution has been determined. In addition, the relative contributions of hydrogen atom transfer, electron transfer and physical deactivation to the overall process of triplet quenching by amino acids have been estimated. Analogous experiments to those with amino acids were carried out with EDTA as the substrate. The results indicate that the flavin triplet state abstracts an electron from EDTA but does not form an excited state flavin-EDTA complex as suggested in a previous study.
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