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Some properties of lipase from Geotrichum asteroides.
Metadata
JournalBiol Bull Acad Sci USSRNot FoundDate
1979-Mar-Apr
Type
Journal Article
Comparative Study
Volume
1979-Mar-Apr / 6 : 261-4
Author
Ksandopulo GB , Ruban EL
Doi
Not Found
PMIDMESH
Catalysis
Geotrichum
Hot Temperature
Hydrogen-Ion Concentration
Kinetics
Lipase
Mitosporic Fungi
Protein Denaturation
Temperature
Abstract
The optimal conditions for the manifestation of the lipase activity of an enzyme preparation from the culture liquid of the fungus Geotrichum asteroides were determined. It was found that the rate of hydrolysis depends on the substrate concentration, the enzyme concentration, and the reaction time. The lipase from G. asteroids is rather stable--it loses practically no activity in a year at 4 degrees C. The temperature optimum of the enzyme preparation is 37 degrees. The lipase from G. asteroides is most active at pH 8.0; it retains its activity when kept for 3 h in a reaction medium with a pH from 3 to 10.
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Biol Bull Acad Sci USSRBiology bulletin of the Academy of Sciences of the USSR
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