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The effect of dietary vitamin E on indomethacin stimulated chemiluminescense in rat liver microsomes.
Metadata
JournalProstaglandins MedNot FoundDate
1979-Jan
Type
Journal Article
Volume
1979-Jan / 2 : 33-42
Author
Pennington SN , Smith CP
DoiPMIDMESH
Animals
Diet
Dose-Response Relationship, Drug
Indomethacin
Lipid Metabolism
Luminescent Measurements
Male
Microsomes, Liver
NADP
Oxidation-Reduction
Rats
Vitamin E
Abstract
Peroxidation of endogenous lipid by rat liver microsomes, coupled with oxidation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) was markedly stimulated in the presence of indomethacin [1-(p-chlorobenzyl)-5-methoxy-2-methyl-3-indole acetic acid], a potent anti-inflammatory drug. This system also generated a rapidly developed chemiluminescense (CL), the intensity and rate of development of which were related to indomethacin concentration and the amount of peroxidation in the sample. Microsomes from rats fed a liquid diet based on Sego diet drink (Pet Incorporated) failed to chemiluminesce with or without added indomethacin. Supplementation of the Sego diet with a polyunsaturated fatty acid (C18:3) did not restore the chemiluminescense. In vitro and in vivo studies indicate that vitamin E (a component of the Sego liquid diet) inhibited the microsomal chemiluminescense reaction in a dose related manner.
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