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A method for the in situ measurement of the electrical quantities in frog skin.
Metadata
JournalJ. Biochem. Biophys. MethodsNot FoundDate
1979-May
Type
Research Support, Non-U.S. Gov't
Journal Article
Volume
1979-May / 1 : 91-104
Author
Bianchi G , Celentano F , Cortili G , Orsenigo MN , Torelli G
DoiPMIDMESH
Animals
Electric Conductivity
Electrophysiology
Hydrogen-Ion Concentration
Rana esculenta
Skin Physiological Phenomena
Temperature
Abstract
A method allowing the measurement of the electrical quantities related to the physiological functions of the frog skin in situ is presented. The method allows the performance of several experiments on the same pithed animal, which remains alive for a number of days. The preparation is very stable, and the electrical potential difference and short-circuit current values are higher than in isolated skin. The theory of measurement and the possible systematic errors are discussed. The possibilities of the method are evaluated on comparing the pH and temperature dependence of the electrical quantities in situ with previous measurements on isolated skin.
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J. Biochem. Biophys. MethodsJournal of biochemical and biophysical methods
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