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Lowered resistance to lytic agents in sucrose vacuolation.
Metadata
JournalVirchows Arch., B, Cell Pathol.Not FoundDate
1979-Dec
Type
Journal Article
Volume
1979-Dec / 32 : 47-56
Author
Benassi G , Sapigni T , Torboli M
DoiPMIDMESH
Animals
Cell Survival
Cells, Cultured
Fibroblasts
Hot Temperature
Mice
Osmolar Concentration
Stress, Mechanical
Sucrose
Surface-Active Agents
Vacuoles
Abstract
Vacuolation in fibroblasts cultivated in the presence of sucrose is associated with progressive accumulation of the undigestible sugar. In radioisotope experiments the process lasted several days, and when the cells were subcultured back into a medium devoid of sucrose the label was also lost after several days. This type of vacuolated cells is more fragile when it is challenged with lytic agents. 51Cr-labelled LS fibroblasts released more radioactivity when they had been growing in the presence of sucrose, whether they were suspended in media of decreasing osmolarity, in dilutions of various surfactants, exposed to high temperatures, or subjected to mechanical stress. It is concluded that these cells exhibit a lower resistance when exposed to unfavourable environments, but retain their viability in growth media despite some morphological and biochemical alterations.
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Virchows Arch., B, Cell Pathol.Virchows Archiv. B, Cell pathology including molecular pathology
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