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[Studies of the antibody heterogeneity in carp (Cyprinus carpio L.). I. Electrophoretic and isoelectric spectra of anti-DNP-antibodies].
Metadata
JournalActa Biol. Med. Ger.Not FoundDate
1979
Type
Journal Article
English Abstract
Volume
1979 / 38 : 1607-14
Author
Machulla HK , Richter RF , Gruhn R , Ambrosius H
Doi
Not Found
PMIDMESH
Animals
Antibodies
Antibody Specificity
Carps
Cyprinidae
Dinitrobenzenes
Haptens
Hydrogen-Ion Concentration
Immunoelectrophoresis
Immunoglobulin M
Isoelectric Focusing
Nitrobenzenes
Abstract
Carp IgM as well as carp anti-DNP-antibodies migrate electrophoretically very well as a diffuse band into polyacrylamide gel of large pore size. The isoelectric spectra of the carp anti-DNP-antibodies are heterogeneous and show bands in the pI-range of 4.0 to 6.4. The activity of focused anti-DNP-antibodies could be demonstrated in the pI range between 5.4 to 6.4 even in high antibody dilutions. The investigated structural heterogeneity of the anti-DNP-antibodies of carp is a further proof for the phylogenetically early onset of a large antibody heterogeneity of lower vertebrates.
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Acta Biol. Med. Ger.Acta biologica et medica Germanica
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