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Regulation of biosynthesis of aminoacyl-transfer RNA synthetases and of transfer-RNA in Escherichia coli.
Metadata
JournalArch Biol Med ExpNot FoundDate
1979-Oct
Type
Research Support, U.S. Gov't, P.H.S.
Journal Article
Volume
1979-Oct / 12 : 415-26
Author
Morgan S , Larossa R , Cheung A , Low B , Söll D
Doi
Not Found
PMIDMESH
Amino Acyl-tRNA Synthetases
Chromosome Mapping
Escherichia coli
Gene Expression Regulation
Genes, Regulator
Glutamate-Ammonia Ligase
Leucine-tRNA Ligase
Mutation
Operon
RNA, Transfer
RNA, Transfer, Amino Acyl
Temperature
Abstract
We have isolated temperature resistant revertants from temperature sensitive E. coli strains containing either a thermolabile glutaminyl-tRNA synthetase or leucyl-tRNA synthetase. Among the revertants which still contained the thermolabile leucyl-tRNA synthetase we found two classes of regulatory mutants (leuX and leu Y) which have elevated levels of this enzyme. The leuX mutation specifies an operator-promoter region adjacent to the structural gene (leuS) for the enzyme. The leuY gene maps away from the leuS gene and codes for a protein. Using these mutants we demonstrated that the levels of leucyl-tRNA are related to the derepression of the leucine and isoleucine-valine operons. Among the revertants which still contained the thermolabile glutaminyl-tRNA synthetase were characterized three classes of mutants, glnT, glnU, and glnR. The glnT and glnU mutants contain elevated levels of tRNAgln, while the glnR mutant possesses elevated levels of glutaminyl-tRNA synthetase. The level of glutamine synthetase, the enzyme responsible for the formation of glutamine, is also derepressed in the glnT and glnR mutants.
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Arch Biol Med ExpArchivos de biologia y medicina experimentales
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