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Microspectrofluorometric studies on the formaldehyde-induced reaction product of 5-hydroxytryptamine.
Metadata
Journaljournal of microscopy1.575Date
1979-Jul
Type
Research Support, Non-U.S. Gov't
Journal Article
Volume
1979-Jul / 116 : 185-98
Author
Schofield GC , Wreford NG
DoiPMIDMESH
Animals
Duodenum
Fluorescence
Formaldehyde
Glycine
Humidity
Hydrogen-Ion Concentration
Male
Mice
Mice, Inbred C3H
Pyloric Antrum
Serotonin
Spectrometry, Fluorescence
Abstract
A new model incorporating Sephadex G-25 as an inert carrier has been developed for studying the reaction between biogenic amines and formaldehyde vapour. Both the fluorescence yield and the spectral characteristics of the 5-HT formaldehyde reaction product are influenced appreciably by the relative humidity at which paraformaldehyde is equilibrated, the duration of the reaction, the glycine concentration and pH of the suspending solution and the duration of exposure to ultraviolet light. It is concluded that care should be exercised in attempting to identify or quantify 5-HT on the basis of microspectrofluorometric analysis.
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1.6
J Microscjournal of microscopy
Metadata
LocationEngland
FromWILEY

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