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Production, purification and properties of a Pichia kluyveri killer toxin.
Metadata
Journalantonie van leeuwenhoek international journal of general and molecular microbiology2.674Date
1979
Type
Journal Article
Volume
1979 / 45 : 437-50
Author
Middelbeek EJ , Hermans JM , Stumm C
DoiPMIDMESH
Ascomycota
Detergents
Fungal Proteins
Glycoproteins
Hot Temperature
Hydrogen-Ion Concentration
Molecular Weight
Mycotoxins
Pichia
Abstract
Production of the killer toxin of Pichia kluyveri 1002 was stimulated in the presence of yeast extract. In a minimal medium production was optimal at pH 3.8-4.0 and 22--25 degrees C. Addition of gelatin and nonionic detergents, like Brij-58 (polyoxyethylene 20 cetyl ether) and Triton-X-100, to this medium enhanced production significantly. The killer toxin was purified 140-fold by use of a stepwise ethanol precipitation and butyl Sepharose column chromatography. The purified killer toxin, which still contained some carbohydrates, appeared to be glycoprotein with a mol wt of about 19 000 and an isoelectric point of 4.3. It was stable between pH 2.5 and 4.7 and up to 40 degrees C.
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Antonie Van Leeuwenhoekantonie van leeuwenhoek international journal of general and molecular microbiology
Metadata
LocationNetherlands
FromSPRINGER

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