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[Analysis of deoxyribonuclease I and II activity as a function of ionic strength and pH].
Metadata
JournalBoll. Soc. Ital. Biol. Sper.Not FoundDate
1979-Oct-15
Type
Journal Article
English Abstract
Volume
1979-Oct-15 / 55 : 2006-11
Author
Gallo A , Sorrentino S , Iorio S , Libonati M
Doi
Not Found
PMIDMESH
Animals
Cattle
Deoxyribonucleases
Hydrogen-Ion Concentration
In Vitro Techniques
Pancreas
Sodium Chloride
Spleen
Swine
Thymus Gland
Abstract
The action of deoxyribonucleases I and II has been studied as a function of ionic strength and pH, in the light of the theory of the ionic control of biochemical reactions (P. Douzou and P. Maurel (1977) Proc. Nat. Acad. Sci. USA, 74, 1013-1015). The pattern of DNA degradation by the two enzymes fits the general principles of the theory. However, the activity of DNAase II, a dimeric, basic protein (pI = 10,2) appears to be scarcely modulated by variables such as ionic strength and pH. This is reminiscent of what was elsewhere observed with the system double stranded RNA-seminal RNAase (also a very basic, dimeric enzyme), and could, therefore, tentatively be correlated with the dimeric and/or the very basic nature of the enzyme protein.
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Boll. Soc. Ital. Biol. Sper.Bollettino della Societa italiana di biologia sperimentale
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