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Relative number and proliferation kinetics of hemopoietic stem cells in the mouse.
Metadata
JournalBlood CellsNot FoundDate
1979-Jun-15
Type
Journal Article
Volume
1979-Jun-15 / 5 : 211-36
Author
Siegers MP , Feinendegen LE , Lahiri SK , Cronkite EP
Doi
Not Found
PMIDMESH
Animals
Bone Marrow Cells
Bone Marrow Transplantation
Cell Count
Cell Division
Female
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Kinetics
Mathematics
Mice
Models, Biological
Abstract
A model calculation of the hemopoiesis of the mouse based on known hematologic data leads to the conclusion that approximately 3% of all nucleated bone marrow cells are stem cells (pluripotent plus committed stem cells). By a new 125IUdR labeling technique on radiation chimeras, a relative number of 2%-7% stem cells was determined. In previous studies with test systems for stem cells using colony formation in vivo or in vitro, a relative number of stem cells of at least one order of magnitude lower has been estimated. In this study the stem cells are found to have a turnover time of about 4.3 days in the donor mice. This turnover time remained unchanged even after transfusion of marrow cells into lethally irradiated recipient mice. Radiosensitivity determinations yielded a D0 of 80 rad for stem cells in S-phase and D0 of 185 rad for stem cells distributed throughout the entire cell cycle. The respective extrapolation numbers were 1.23 and 1.14. Experiments using an 3H-TdR suicide technique revealed different cell cycle parameters for bone marrow stem cells seeding to the spleens and to the femurs of lethally irradiated recipients, primarily a shortening of S-phase in cells seeding to femurs. The method described here provides a new approach to hematologic stem cell research.
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