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The quantum yield of flash-induced proton release by bacteriorhodopsin-containing membrane fragments.
Metadata
Journalbiophysical journal3.854Date
1979-Feb
Type
Research Support, U.S. Gov't, Non-P.H.S.
Journal Article
Volume
1979-Feb / 25 : 341-53
Author
Ort DR , Parson WW
DoiPMIDMESH
Bacteriorhodopsins
Carotenoids
Halobacterium
Hydrogen-Ion Concentration
Kinetics
Light
Mathematics
Osmolar Concentration
Quantum Theory
Spectrophotometry
Abstract
The quantum yield of proton release by bacteriorhodopsin was measured from volume changes after excitation of purple membrane fragments by short flashes. At low ionic strengths, about 0.25 mol of protons is released per einstein absorbed. This agrees well with quantum yields reported recently for the conversion of bacteriorhodopsin into a metastable state (M) that absorbs near 412 nm. However, the quantum yield of proton release increases gradually with increasing ionic strength; it plateaus with a value of 0.43 +/- 0.03 at ionic strengths above 200 mM. Changing the ionic strength has no detectable effect on the quantum yield of formation of the M spectral state. It thus appears that as many as two protons can be released and rebound in each photochemical cycle at high ionic strengths. The quantum yield of proton release is essentially independent of pH over the range 6.0-8.75. The quantum yield decreases with increasing flash strength, apparently due to photoreversal of the initial photochemical reaction.
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3.9
Biophys Jbiophysical journal
Metadata
LocationUnited States
FromCELL PRESS

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