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Bacteriorhodopsin in model membranes. A new component of the displacement photocurrent in the microsecond time scale.
Metadata
Journalbiophysical journal3.854Date
1979-Mar
Type
Research Support, U.S. Gov't, P.H.S.
Research Support, Non-U.S. Gov't
Journal Article
Volume
1979-Mar / 25 : 465-72
Author
Hong FT , Montal M
DoiPMIDMESH
Bacteriorhodopsins
Carotenoids
Electric Conductivity
Halobacterium
Hydrogen-Ion Concentration
Kinetics
Membranes, Artificial
Models, Biological
Temperature
Time Factors
Abstract
A quasi-short-circuit (tunable voltage clamp) measurement method with microsecond time resolution was applied to a bacteriorhodopsin model membrane formed by a novel interfacial technique. A new component (B1) of the displacement photocurrent was recorded: it has no detectable latency at an instrumental time constant of 1.5 museconds, and persists at 5 degrees C. In addition, a slower component (B2) of opposite polarity inhibited by low temperature (5 degrees C) and low pH (pH = 3.0) was recorded. The technique is very sensitive for the study of fast capacitative photoresponses in model membranes, and allows the detection of charge displacements in bacteriorhodopsin associated with distinct stages of the photochemical transformation.
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3.9
Biophys Jbiophysical journal
Metadata
LocationUnited States
FromCELL PRESS

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