MedicGo
Nuclear magnetic resonance determinations of permeation coefficients for maleic acid in phospholipid vesicles.
Metadata
Journalbiophysical journal3.854Date
1979-Jun
Type
Research Support, U.S. Gov't, P.H.S.
Journal Article
Volume
1979-Jun / 26 : 575-84
Author
Prestegard JH , Cramer JA , Viscio DB
DoiPMIDMESH
Cholesterol
Hydrogen-Ion Concentration
Lipid Bilayers
Magnetic Resonance Spectroscopy
Maleates
Mathematics
Models, Biological
Permeability
Phosphatidylcholines
Valinomycin
Abstract
Lipid bilayer permeation coefficients for the neutral maleic acid molecule and the maleate monoanion have been determined by proton magnetic resonance techniques. Phosphatiydylcholine-cholesterol (2:1) unilamellar vesicles were prepared having an initial maleate anion concentration gradient stabilized by coupling to an impermeant potassium counterion. The coupling was released by addition of valinomycin, and the time evolution of external pH, internal pH, and maleate concentration followed using nuclear magnetic resonance areas and chemical shifts. Transport rate equations were numerically integrated to fit the date, yielding best fit permeation coefficients of 4 X 10(-9) and 4 X 10(-5) cm/s for maleate monoanion and maleic acid, respectively.
Fav
Like
Download
Share
Export
Cite
3.9
Biophys Jbiophysical journal
Metadata
LocationUnited States
FromCELL PRESS

No Data

© 2017 - 2020 Medicgo
Powered by some medical students