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Viscoelastic behavior of mammalian DNA.
Metadata
Journalbiophysical journal3.854Date
1979-Oct
Type
Research Support, U.S. Gov't, P.H.S.
Research Support, Non-U.S. Gov't
Journal Article
Volume
1979-Oct / 28 : 93-105
Author
Chase ES , Shafer RH
DoiPMIDMESH
Animals
Brain Neoplasms
Cell Line
DNA
Elasticity
Hydrogen-Ion Concentration
Kinetics
Neoplasms, Experimental
Nucleic Acid Conformation
Rats
Viscosity
Abstract
The viscoelastic behavior of rat 9L cellular DNA was studied as a function of the detergent used for lysis, the pH and duration of lysis, and gamma ray dose. For nondenaturing lysis conditions, a model of the DNA was proposed to account for the effects of these agents on the viscoelastic retardation time. It was concluded that these agents affect the hydrodynamic radius of the DNA rather than its molecular weight. For denaturing lysis conditions, molecular weights calculated from the relaxation time were consistent with those calculated from alkaline sucrose sedimentation profiles.
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3.9
Biophys Jbiophysical journal
Metadata
LocationUnited States
FromCELL PRESS

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