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Isolation, cryopreservation, and autotransplantation of human stem cells.
Metadata
Journalexperimental hematology2.82Date
1979
Type
Research Support, Non-U.S. Gov't
Journal Article
Volume
1979 / 7 Suppl 5 : 12-20
Author
Wells JR , Ho WG , Graze P , Sullivan A , Gale RP , Cline MJ
Doi
Not Found
PMIDMESH
Bone Marrow Cells
Cell Separation
Colony-Forming Units Assay
Freezing
Hematopoietic Stem Cell Transplantation
Humans
Preservation, Biological
Abstract
We have used cryopreserved autologous hematopoietic cells to repopulate bone marrow of patients receiving intensive chemotherapy and radiation. We employed the technique of density step centrifugation to concentrate stem cells from whole bone marrow. The concentrate of cells was frozen in vials using dimethyl sulphoxide (DMSO) as a cryo-protective agent and was stored in the vapor phase of liquid nitrogen. Test vials were thawed and assayed for myeloid stem cells (CFU-C) to determine the potential of the larger marrow aliquot. These test values were compared to the recovery of cells and CFU-C when the marrow was used for autotransplantation. The return of hematopoietic function after autotransplantation was evaluated and correlated with the dosage of cells and CFU-C infused.
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2.8
Exp Hematolexperimental hematology
Metadata
LocationNetherlands
FromELSEVIER SCIENCE INC

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