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A comparison of cold and acid activation of big renin and of inactive renin in normal plasma.
Metadata
Journaljournal of clinical endocrinology & metabolism5.399Date
1978-Oct
Type
Research Support, U.S. Gov't, P.H.S.
Journal Article
Comparative Study
Volume
1978-Oct / 47 : 792-9
Author
Hsueh WA , Luetscher JA , Carlson E , Grislis G , Elbaum D , Chavarri M
DoiPMIDMESH
Amniotic Fluid
Chromatography, Gel
Cold Temperature
Diabetic Nephropathies
Enzyme Activation
Enzyme Precursors
Female
Humans
Hydrogen-Ion Concentration
Pregnancy
Renin
Abstract
Normal human plasma contains "inactive renin," whose ability to generate angiotensin I increases after exposure to pH 3.3. Big renin is a partially inactive enzyme of larger molecular weight, which is also activated at pH 3.3, and is found of pregnant women, and in amniotic fluid, but not in normal plasma. We have compared the effects of acid exposure and storage at 4 and -4 C on normal plasma and plasma containing big renin. The concentration of inactive renin in normal plasma was approximately equal to that of normal active renin, and its activity increased slowly on prolonged standing at -4 but not 4 C. In contrast, the activity of big renin increased by 50% as early as 1-3 days at 4 C and increased even more quickly at -4 C. Acid treatment of plasma containing big renin caused 4-10 times greater increase in active renin than similar treatment of normal plasma. During gel filtration, both cold-activated and previously acidified big renin coeluted with unactivated big renin. These data indicate that big renin is highly susceptible to cold or acid activation and that such activation of big renin does not result in a detectable decrease in its molecular weight of 60,000 daltons. Furthermore, acid and cold seem to activate the same pool of inactive renin in normal plasma. Although both normal and big renin are stable for long periods below -20 C, a serious overestimate of plasma renin activity can occur if plasma is stored just above its freezing point before assay.
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5.4
J Clin Endocrinol Metabjournal of clinical endocrinology & metabolism
Metadata
LocationUnited States
FromENDOCRINE SOC

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