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Head-to-head association in bovine spermatozoa induced by catecholamines.
Metadata
Journalexperimental cell research3.383Date
1978-May
Type
Research Support, Non-U.S. Gov't
Journal Article
Volume
1978-May / 113 : 421-33
Author
Lindahl PE 1
Affiliation

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Doi
PMIDMESH
3',5'-Cyclic-AMP Phosphodiesterases
Adrenergic alpha-Antagonists
Adrenergic beta-Antagonists
Adrenochrome
Animals
Bucladesine
Calcium
Catecholamines
Cattle
Cell Aggregation
Cyclic AMP
Male
Sperm Head
Spermatozoa
Abstract
Noradrenaline, adrenaline, and isoproterenol induce head-to-head association in bovine spermatozoa in a Tris-HCl-buffered medium. Noradrenaline was the most and isoproterenol the least efficient. This effect was stimulated by Ca2+, Mg2+ and Mn2+, Ca2+ being more efficient than the other two ions. At 2 X 10(-6) M Ca2+, oxidation products of adrenaline dissociated spermatozoa associated by washing; at 2 X 10(-5) M Ca2+, the dissociating effect was transformed into association. The induction of association by adrenaline was blocked by both alpha- and beta-adrenergic blockers at low concentrations (2 X 10(-7) M). Both cAMP and dibutyryl substituted cAMP (db-cAMP) induced association in the Tris-buffered medium at 2 X 10(-6) M Ca2+. Further increase in association was brought about by increasing the Ca2+ concentration to 2 X 10(-5) M. Prolongation of the treatment with cAMP increased association. When combined with cAMP under the same conditions as used in the combination with adrenaline, L-propranolol did not inhibit association induced by cAMP. In an identical experiment, performed in Tyrode solution, L-propranolol inhibited association induced by cAMP. At 2 X 10(-5) M, theophylline, caffeine, and papaverine induced association in the presence of 2 X 10(-5) M Ca2+. The results are compatible with the hypothesis that catecholamines act via receptors and formation of cAMP.
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3.4
Exp Cell Resexperimental cell research
Metadata
LocationUnited States
FromELSEVIER INC

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