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A sensitive radiometric assay for cysteic acid decarboxylase activity in crude enzyme preparations of rat liver and brain.
Metadata
JournalComp. Biochem. Physiol., BNot FoundDate
1977
Type
Research Support, Non-U.S. Gov't
Journal Article
Volume
1977 / 58 : 115-7
Author
Yam CF 1, Tunnicliff G , Ngo TT , Barbeau A
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Doi
PMIDMESH
Animals
Brain
Carboxy-Lyases
Cysteic Acid
Hydrogen-Ion Concentration
Liver
Male
Radiometry
Rats
Rats, Inbred Strains
Substrate Specificity
Abstract
1. A method is described for the synthesis of L-[U-14C]cysteic acid from L-[U-14C] cysteine hydrochloride and for its subsequent utilisation as a substrate for cysteic acid decarboxylase activity in liver and brain. 2. The enzyme determination relies on the entrapment of radio-labelled carbon dioxide in Hyamine hydroxide. 3. The assay is sensitive, reliable and convenient and is particularly suitable for measuring the activity of the decarboxylase in crude enzyme preparations.
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Comp. Biochem. Physiol., BComparative biochemistry and physiology. B, Comparative biochemistry
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