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Hexokinase of the adult dog heartworm, Dirofilaria immitis.
Metadata
JournalComp. Biochem. Physiol., BNot FoundDate
1977
Type
Journal Article
Volume
1977 / 58 : 131-4
Author
Hutchison WF 1, Turner AC , Oelshlegel FJ
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Doi
PMIDMESH
Animals
Dirofilaria immitis
Dogs
Electrophoresis, Starch Gel
Enzyme Stability
Filarioidea
Glucosephosphates
Hexokinase
Hydrogen-Ion Concentration
Isoenzymes
Substrate Specificity
Temperature
Abstract
1. Dirofilaria immitis hexokinase was relatively heat stable and had a pH optimum range between 7.8 and 8.2. 2. Mean Vmax was 0.40 +/- 0.10 (S.D.) mumole/min/100 mg of worm. 3. Mean Km values were 0.32 mM for glucose, 0.86 mM for fructose and 0.39 mM for ATP. 4. Glucose-6-phosphate was not a strong product inhibitor. 5. Starch gel electrophoresis demonstrated at least three isozymes.
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Comp. Biochem. Physiol., BComparative biochemistry and physiology. B, Comparative biochemistry
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