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Automatic measurement of the oxygen affinity of Cancer magister hemocyanin.
Metadata
JournalComp. Biochem. Physiol., BNot FoundDate
1977
Type
Research Support, U.S. Gov't, P.H.S.
Research Support, Non-U.S. Gov't
Journal Article
Volume
1977 / 57 : 139-41
Author
Wajcman H 1, McMahill P , Mason HS
Affiliation

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Doi
PMIDMESH
Animals
Binding Sites
Brachyura
Calcium
Hemocyanins
Hemolymph
Hydrogen-Ion Concentration
Magnesium
Oxygen
Temperature
Abstract
1. The P50 of Cancer Magister hemocyanin in hemolymph was 40.0 mm at 25 degrees C, nmax = 3.9; at 14 degrees C, 15.0 mm and 3.4; and at 10 degrees C. 10.2 mm and 3.3. 2. In the absence of Ca2+ and Mg2+, the P50 at 25 degrees C was in the range of 150 to 250 mm, depending upon pH. 3. Our results show that temperature variation and pH variation may be important factors in the physiological regulation of oxygen delivery in this species. 4. The automatic recording device of Imai et al. (1970) previously used for the study of hemoglobins, was found to be eminently suitable for the study of O2 affinities of hemocyanins.
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Comp. Biochem. Physiol., BComparative biochemistry and physiology. B, Comparative biochemistry
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