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Decreased UV mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae.
Metadata
JournalMol. Gen. Genet.Not FoundDate
1979
Type
Research Support, U.S. Gov't, P.H.S.
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Journal Article
Volume
1979 / 172 : 249-58
Author
Prakash L 1, Hinkle D , Prakash S
Affiliation

No Data

Doi
PMIDMESH
Alleles
DNA Replication
DNA, Fungal
DNA-Directed DNA Polymerase
Dose-Response Relationship, Radiation
Genes, Fungal
Mutagenesis
Radiation Tolerance
Saccharomyces cerevisiae
Ultraviolet Rays
Abstract
A DNA replication mutant of yeast, cdc8, was found to decrease UV-induced reversion of lys2-1, arg4-17, tyr1 and ura1. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following UV irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as well as to rad1; cdc8 rad51 double mutants seem to be more sensitive than the single mutants. Since UV-induced reversion in cdc8 rad1 and cdc8 rad51 double mutants is like that of the cdc8 single mutants, we conclude that CDC8 plays a direct role in error-prone repair. To test whether CDC8 codes for a DNA polymerase, we have purified both DNA polymerase I and DNA polymerase II from cdc8 and CDC+ cells. The purified DNA polymerases from cdc8 were no more heat labile than those from CDC+, suggesting that CDC8 is not a structural gene for either enzyme.
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Mol. Gen. Genet.Molecular & general genetics : MGG
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