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Proton conductor vs. cold in induction of Ca(2+)-dependent competence in Escherichia coli.
Metadata
JournalMol. Gen. Genet.Not FoundDate
1979
Type
Journal Article
Volume
1979 / 172 : 313-7
Author
Sabelnikov AG 1, Domaradsky IV
Affiliation

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Doi
PMIDMESH
Calcium
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Cold Temperature
DNA, Bacterial
Drug Resistance, Microbial
Escherichia coli
Oxidative Phosphorylation
Permeability
Protons
Transformation, Bacterial
Uncoupling Agents
Abstract
In order to elucidate the molecular mechanisms of Ca(2+)-dependent competence in gram-negative bacteria an attempt was made to induce the competence at room temperature in presence of a proton conductor, carbonylcyanide-m-chlorophenylhydrazone (CCCP). Escherichia coli K12 cells treated with Ca2+ at 25 degrees or 37 degrees C in presence of CCCP became permeable for transforming plasmid and transfecting DNAs and DNA-binding antibiotic actinomycin C (AmC) and rubomycin (Rm) at room temperature. The efficiencies of transformation and transfection, however, were by 1-3 orders of magnitude lower compared to cells, treated with Ca2+ at 0 degree C, though both recipients did not differ significantly in their susceptibility to AmC and Rm. Possible mechanisms of Ca2+ action in both recipient systems are discussed in terms of molecular interactions.
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Mol. Gen. Genet.Molecular & general genetics : MGG
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