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Bile acids. XLVII. 12alpha-Hydroxylation of precursors of allo bile acids by rabbit liver microsomes.
Metadata
JournalBiochim. Biophys. ActaNot FoundDate
1975-Nov-21
Publication Type
Research Support, U.S. Gov't, P.H.S.
Journal Article
Volume
1975-Nov-21 / 409 : 249-57
Author
Ali SS , Elliott WH
DoiPMIDMESH
Aging
Animals
Bile Acids and Salts
Female
Kinetics
Male
Microsomes, Liver
Mixed Function Oxygenases
NADP
Oxidation-Reduction
Rabbits
Sex Factors
Abstract
Rabbit liver microsomal preparations fortified with 0.1 mM NADPH effectively promote hydroxylation of [3beta-3H]- or [24-14C]allochenodeoxycholic acid or [5alpha,6alpha-3H2]5alpha-cholestane-3alpha,7alpha-diol to their respective 12alpha-hydroxyl derivatives in yields of about 25 or 65% in 60 min. Minor amounts of other products are formed from the diol. The requirements for activity of rabbit liver microsomal 12alpha-hydroxylase resemble those of rat liver microsomes. Of a number of enzyme inhibitors studied only p-chloromercuribenzoate demonstrated a marked ability to inhibit the reaction with either tritiated substrate. There was no difference in the quantity of product produced from the tritiated acid or the 14C-labeled acid. No clear sex difference was found in activity of the enzyme, nor was an appreciable difference noted in activity of the enzyme between mature and immature animals.
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